Zinc Finger–Based Knockout Punches for Zebrafish Genes
نویسندگان
چکیده
منابع مشابه
Delivering Three Punches to Knockout Intracellular Bacteria
Cytotoxic lymphocytes kill bacteria-infected cells, but the mechanisms at work remain unclear. Walch et al. show that these lymphocytes deliver a toxic molecular trio in a two-step process, penetrating first the infected cells and then delivering bactericidal granzymes into the intracytoplasmic bacteria.
متن کاملEmerging gene knockout technology in zebrafish: zinc-finger nucleases.
One advantage of the zebrafish model system is the ability to use forward genetics to reveal critical gene functions by their mutant phenotype. Reverse genetic tools are available, although it is more challenging and time-consuming to identify mutations in specific genes of interest and virtually impossible to induce mutations in a targeted manner. Two recent papers have shown that locus-specif...
متن کاملBig punches come in nanosizes for chemoprevention.
Literature to support the chemopreventive potential of several bioactive molecules has been prolific and convincing, but the clinical development of these agents has been slow. Major hurdles for development of bioactive chemoprevention approaches include low potency, lack of reliable formulations with high bioavailability that are suitable for oral administration, and relevant preclinical prima...
متن کاملGeneration and Characterization of Fmr1 Knockout Zebrafish
Fragile X syndrome (FXS) is one of the most common known causes of inherited mental retardation. The gene mutated in FXS is named FMR1, and is well conserved from human to Drosophila. In order to generate a genetic tool to study FMR1 function during vertebrate development, we generated two mutant alleles of the fmr1 gene in zebrafish. Both alleles produce no detectable Fmr protein, and produce ...
متن کاملRapid Mutation of Endogenous Zebrafish Genes Using Zinc Finger Nucleases Made by Oligomerized Pool ENgineering (OPEN)
BACKGROUND Customized zinc finger nucleases (ZFNs) form the basis of a broadly applicable tool for highly efficient genome modification. ZFNs are artificial restriction endonucleases consisting of a non-specific nuclease domain fused to a zinc finger array which can be engineered to recognize specific DNA sequences of interest. Recent proof-of-principle experiments have shown that targeted knoc...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Zebrafish
سال: 2008
ISSN: 1545-8547,1557-8542
DOI: 10.1089/zeb.2008.9988